| Genes | Primers (5’-3’) | Annealing temp (˚C) | Amplicon size (bp) | ||
| POU5F1 (Oct-4) | F | GCAATTTGCCAAGCTCCTGAAGCAG | 55 | 536 | |
| R | CATAGCCTGGGGTACCAAAATGGGG | ||||
| NANOG (Nanog) | F | GGTGGCAGAAAAACAACTGGC | 55 | 300 | |
| R | TGCAGGACTGCAGAGATTCC | ||||
| TERT (hTERT) | F | GCAGCTCCCATTTCATCAGC | 53 | 343 | |
| R | CAGGATGGTCTTGAAGTCTG | ||||
| AFP | F | CAGAAAAATGGCAGCCACAGC | 54 | 399 | |
| R | TGGCAGCATTTCTCCAACAGG | ||||
| ACTC1 | F | CATCCTGACCCTGAAGTATCCCATC | 56 | 315 | |
| R | CCCTCATAGATGGGGACATTGTGAG | ||||
| SOX1 | F | CCAGGAGAACCCCAAGAGGC | 56 | 206 | |
| R | CGGCCAGCGAGTACTTGTCC | ||||
| ACTB (β-actin) | F | GCCACGGCTGCTTCCAGC | 55 | 477 | |
| R | AGGGTGTAACGCAACTAAGTC | ||||